Integrating the DNA Integrity Number (DIN) to Assess Genomic DNA (gDNA) Quality Control Using the Agilent 2200 TapeStation System Authors
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چکیده
Next Generation Sequencing (NGS) requires the input of high molecular weight genomic DNA (gDNA) to construct quality libraries for large scale sequencing projects, such as the 100K Pathogen Genome Project. The assessment of DNA integrity is a critical first step in obtaining meaningful data, and intact DNA is a key element for successful library construction. The Agilent 2200 TapeStation System plays an important role in the determination of the DNA quality using the DNA genomic assay. Profiles generated on the 2200 TapeStation System yield information on concentration, allow a visual inspection of the DNA quality, and generate a DNA Integrity Number (DIN), which is a value automatically assigned by the software that provides an indication of integrity (that is, lack of degradation). This application note describes a new software algorithm that has been developed to extract information about DNA sample integrity from the 2200 TapeStation System electrophoretic trace. The Agilent 2200 TapeStation System
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تاریخ انتشار 2016